熱門關鍵詞:蘇瑪(ma)罐,原子(zi)吸(xi)收(shou)分(fen)光光度計,環(huan)境(jing)監測產品(pin),液(ye)相色譜,色譜柱。
更(geng)新時(shi)間:2019-11-12
CarboPac PA200碳水化合物色譜柱對于高分辨率測(ce)繪和(he)糖(tang)蛋白釋放的寡糖(tang)的分析
免費(fei)咨詢:010-61755520
對于高分辨率測繪和糖(tang)(tang)蛋(dan)白釋放的(de)寡糖(tang)(tang)的(de)分析(xi),使(shi)用的(de)Thermo Scientific戴安(an)CARBOPACPA200分析(xi)及保護(hu)柱提(ti)供的(de)敏(min)感性(xing)和特異(yi)性(xing)檢測。脈沖安(an)培檢測(PAD)相結(jie)合(he)(he),此陰(yin)離(li)(li)子交(jiao)換(huan)柱提(ti)供無需使(shi)用衍生(sheng)快速,重復分離(li)(li)。疏水性(xing)聚合(he)(he)物,薄(bo)殼(ke)型陰(yin)離(li)(li)子交(jiao)換(huan)樹脂填(tian)充材料是在(zai)pH值(zhi)0-14的(de)范圍內保持穩(wen)定(ding)。這(zhe)種(zhong)*的(de)pH值(zhi)穩(wen)定(ding)性(xing)允許使(shi)用有利于碳水化合(he)(he)物的(de)陽極(ji)氧化的(de)金電極(ji)洗脫(tuo)液組成的(de)。
CarboPac PA200碳水化合物色譜柱
訂貨信息:
Dionex CarboPac PA200 碳水化合物分析柱
分析柱 | |
CarboPac PA200 Analytical Column (3 x 250 mm) | 062896 |
保護柱 | |
CarboPac PA200 Guard Column (3 x 50 mm) | 062895 |
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
---|---|
Dimensions | CarboPac? PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm |
Maximum operating pressure | 4000 psi (27.9 MPa) |
Temperature range | 6–60 °C Recommended operating temperature: controlled ambient |
Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
Resin composition | 5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked) |
Anion-exchange capacity | Approximately 35 μeq/column (3 × 250 mm analytical column) |
Column construction | PEEK? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |