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Dionex CarboPac PA1 糖和氨基酸色譜柱

更(geng)新時間(jian):2019-11-14

簡要描述:

Dionex CarboPac PA1 糖和氨基酸色譜柱
這款的陰(yin)離子交換柱與脈沖電流(liu)檢測技術 (PAD) 結合使(shi)用,可提供高分辨率的分離。

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Dionex CarboPac PA1 糖和氨基酸色譜柱

對于各種基質(zhi)中單糖、雙糖和(he)線性多(duo)糖的(de)測(ce)定,使用耐用的(de) Thermo Scientific? Dionex? CarboPac? PA1 分析和(he)保護柱。 這(zhe)款的(de)陰離(li)子交換柱與脈沖(chong)電(dian)流(liu)檢測(ce)技術 (PAD) 結(jie)合(he)使用,可提供(gong)(gong)高(gao)分辨率的(de)分離(li)。 Dionex CarboPac PA1 樹脂的(de)* Thermo Scientific? Dionex? MicroBead? 薄殼結(jie)構,使其(qi)在所(suo)有緩沖(chong)鹽溶液(ye)中可保持 pH 0–14 的(de)穩定性。 薄殼的(de)樹脂結(jie)構提供(gong)(gong)出色的(de)質(zhi)量傳(chuan)遞優秀(xiu)的(de)傳(chuan)質(zhi),從而實現快速的(de)梯度再平(ping)衡(heng)。

 

 

描述

用于碳水化合物分離的耐用型柱

  • 經驗證適(shi)用于各種正式(shi)的食(shi)物分析方(fang)法(fa)

  • 特別(bie)適合針(zhen)對營養(yang)標簽進行的食物分(fen)析(xi)

  • 非常適用于中性與酸性單糖的分離

  • 靈敏度低于 Dionex CarboPac PA10 或 PA20 柱

  • 提供微(wei)孔、標準孔和半制備規格

關于 Dionex CarboPac 柱系列

Dionex CarboPac 系列提供精選(xuan)的(de)高性能液相(xiang)色譜(pu) (HPLC) 柱,每一款均為不(bu)同類別化(hua)合物(wu)而(er)優化(hua)。 與能夠檢測碳水化(hua)合物(wu)而(er)不(bu)產生衍生物(wu)的(de) PAD 配合使用,這(zhe)些色譜(pu)柱支(zhi)持(chi)簡(jian)單可靠的(de)糖(tang)(tang)醇、單糖(tang)(tang)和(he)雙糖(tang)(tang)、多糖(tang)(tang)和(he)低聚糖(tang)(tang)分離技術(shu)。

Dionex CarboPac 柱提供以下成分的高分辨率分離:

  • 膳食纖維中(zhong)的糖(tang)蛋(dan)白單糖(tang)和復合碳水化(hua)合物,包(bao)括果聚(ju)糖(tang)、麥芽糊精(jing)和支鏈淀粉

  • 單糖(tang),例如從糖(tang)蛋白(bai)水(shui)解(jie)物和植物水(shui)解(jie)物得到的單糖(tang)

  • 單糖和雙糖

 

用途和特點
AminoPac PA1柱用于(yu)氨(an)基(ji)酸的快速分(fen)(fen)離。特別適用于(yu)分(fen)(fen)析酸性(xing)(xing)和酸不(bu)穩定性(xing)(xing)氨(an)基(ji)酸,以及用陽離子交換分(fen)(fen)離不(bu)*的氨(an)基(ji)酸對。

 

Dionex CarboPac PA1 糖和氨基酸色譜柱

訂貨信息:

Dionex CarboPac PA1 糖和氨基酸分析柱

分析柱(zhu)
CarboPac PA1 Analytical Column (2 x 250 mm)057178
CarboPac PA1 Analytical Column (4 X 250 mm)035391
CarboPac PA1 Semi-Preparative Column (9 x 250 mm)039686
CarboPac PA1 Semi-Preparative Column (22 x 250 mm)0SP2866
保護柱
CarboPac PA1 Guard Column (2 x 50 mm)057179
CarboPac PA1 Guard Column (4 x 50 mm)043096
CarboPac PA1 Guard (9 x 50 mm)063501

 

 

 

Ideal Column for the Separation of Neutral and Acidic Monosaccharides

The CarboPac? PA1 is an anion-exchange column for the separation of mono-, disaccharides, and specific oligosaccharides using an isocratic eluent. Sialic acid analysis has also been demonstrated on this column. 

  • General-purpose column for isocratic determination of monosaccharides and disaccharides

  • Detection by pulsed amperometric detection; no derivatization needed

  • Approved for use in a variety of official methods for the analysis of foods

The unique MicroBead? pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration.

The CarboPac PA1 column is used with pulsed amperometric detection; no derivatization is required. Sensitivity on the PA1 is not as high as with the CarboPac PA20 or PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods.

 

 

CarboPac PA1 Column for Routine Carbohydrate Analysis  Specifications
DimensionsCarboPac PA1 Analytical Column:
4 × 250 mm and 2 × 250 mm
CarboPac PA1 Guard Column:
4 × 50 mm and 2 × 50 mm
CarboPac PA1 Semipreparative Column:
22 × 250 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range4–55 °C
Recommended operating temperature: ambient
Typical operating conditions1500 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min (for 4 × 250 mm)
Mobile phase compatibilitypH 0–14; up to 2% of common HPLC solvents.
Acetate or hydroxide eluents only.
Resin composition10-μm-diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500-nm MicroBead quaternary ammonium functionalized latex (5% cross-linked).
Anion-exchange capacityApproximately 100 μeq/column (4 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded-ferrule-style end fittings.
All components are nonmetallic.

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